Developing Chromosome Segment Substitution Lines with Xiushui 134 as Recurrent Parent and Yangdao 6 as Donor Parent

doi:10.3969/j.issn.1006-8082.2019.02.007

Abstract

Abstract:

Chromosome segment substitution lines （CSSLs）, a powerful tools for identifying favorable alleles in unadapted germplasm, is being widely used. In this study, the authors developed a set of CSSL population consisting of 53 lines which were derived from the recipient Xiushui 134 and the dono Yangdao 6. The CSSL length of substitution segments ranged from 7.4 to 182.15 cM, it accounted averagely for 5.0% （from 0.49% to 12.15%） of whole rice genome. The total length of substitution segments including 12 chromosomes was 1 995 cM, which was 1.33 times as long as the whole rice genome. In conclusion, strategy for developing CSSL and its application were discussed.

Key words: rice, molecular marker, genetic linkage map, chromosome segment substitution lines （CSSLs）

摘要：

染色体片段代换系作为发掘和鉴定有利基因的有力工具得到了广泛的应用。本研究以秀水134为背景亲本，以扬稻6号为供体亲本，构建了一套染色体片段代换系。该代换系包含53个株系，其代换片段长度在7.4~182.15 cM之间，占水稻全基因组的0.49%~12.15%，平均占水稻全基因组的5.0%。这套代换系的代换片段总长度为1 995 cM，覆盖水稻基因组的1.33倍。本文还对染色体片段代换系的构建策略和应用进行了讨论。

关键词: 水稻, 分子标记, 遗传连锁图谱, 染色体片段代换系

CLC Number:

S511

孟卓玲#,曹玉洁#,单丹丹,方春,缪小菲,费月新,方柄杰,王越,赵文佳,侯璐燕,吴敏,吴洪恺*. 秀水134为背景的染色体片段代换系的构建[J]. 中国稻米, DOI: 10.3969/j.issn.1006-8082.2019.02.007 .

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